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KMID : 0359319860260020245
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Korean Journal of Veterinary Research
1986 Volume.26 No. 2 p.245 ~ p.249
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Phytomitogen Induced Blast Transformation of Guinea Pig
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Abstract
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The present study has been carried out to investigate the optimal condition on the blastogenesis of guinea pig lymphocytes. A microculture system in conjunction with a semiautomatic multiple sample harvester(SAMSH) was used to study the in vito optimal condition of guinea pig lymphocytes.
Data were presented to show many variables that are Involved in studying the responses of guinea pig lymphocyte in a microculture system to the stimulation of Concanavalin A(Con A) and lipopolysaccharide (LPS) .
Analysis indicated that the conditions for optimal Con A as measured by incorporation of ³H-TdR include :
(1) use of RPMI-1640 as culture medium,
(2) use of 6㎍ of Con A, per culture,
(3) use of 1×10＾6 cells per culture.
Conditions for optimal stimulation with LPS mitogen were similar to those used for Con A.
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